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MySQL Error: 1194 (Table 'dev_comment' is marked as crashed and should be repaired)
#0 dbbase_sql->halt(Invalid SQL: select count(id) from dev_comment where pid='159795' and iffb='1') called at [/data/www/shop/includes/db.inc.php:54] #1 dbbase_sql->query(select count(id) from {P}_comment where pid='159795' and iffb='1') called at [/data/www/shop/comment/module/CommentContent.php:65] #2 CommentContent() called at [/data/www/shop/includes/common.inc.php:551] #3 printpage() called at [/data/www/shop/comment/html/index.php:13]
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Warning: mysql_query() [function.mysql-query]: Unable to save result set in /data/www/shop/includes/db.inc.php on line 50
Database error: Invalid SQL: select * from dev_comment where pid='159795' and iffb='1' order by id limit 0,10
MySQL Error: 1194 (Table 'dev_comment' is marked as crashed and should be repaired)
#0 dbbase_sql->halt(Invalid SQL: select * from dev_comment where pid='159795' and iffb='1' order by id limit 0,10) called at [/data/www/shop/includes/db.inc.php:54] #1 dbbase_sql->query(select * from {P}_comment where pid='159795' and iffb='1' order by id limit 0,10) called at [/data/www/shop/comment/module/CommentContent.php:167] #2 CommentContent() called at [/data/www/shop/includes/common.inc.php:551] #3 printpage() called at [/data/www/shop/comment/html/index.php:13]
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发布于:2019-3-23 15:53:52  访问:74 次 回复: 篇
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F any observed interactions, {another|an additional|yet another|one more
We assume this to be a non-specific artifact considering that it occurs with all 3 kinase constructs and does not happen in the opposite vector combination. (5) Full length YycG but not YycG211-611 interacts with FtsW in one vector mixture. (6) YycG and YycG211-611 interact with EzrA in 1 but not the other vector mixture. The information suggests that YycG tends to make specific interactions with FtsL, DivIB, Pbp2B and interactions with FtsW and EzrA appear also achievable. Considering that all of those interactions except the FtsW interaction are PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/19373244 also observed with the YycG211-611 fragment, these interactions likely happen through the transmembrane and extra-cytoplasmic domains of YycG. We can‘t dismiss the possibility that some of these interactions are indirect, mediated by way of a popular E. coli divisome interaction companion.watermark-text watermark-text watermark-textDeletion or depletion of later stage cell division proteins doesn‘t Bay 43-9006 medchemexpress perturb YycG localization The two-hybrid assay identified powerful interactions in between YycG and also the cell division proteins DivIB, FtsL and Pbp2B along with a possible interaction with FtsW. We previously showed that YycG fails to localize in a strain depleted for FtsZ (Fukushima et al., 2008). To assess whether or not any other person proteins were required for YycG localization towards the septum and thereby prove the physiological relevance from the two-hybrid outcomes we wanted to probe YycG localization in strains that were either deleted for non-essential cell division proteins or that harbored vital cell division genes under handle with the IPTG inducible Pspac or the xylose inducible Pxyl Vandetanib inhibitor promoter to permit for depletion of the proteins upon removal on the inducer. Strains tested had been (a) a temperature sensitive divIC strain (JH25070), (b) divIB (JH25072), (c) Pspac-ftsA/sepF (YK200), (d) Pspac-ftsW (FtsWP), (e) Pspac-pbpB/Pxyl-ftsL (804) (f) Pspac- pbpB/Pxyl-ftsL/divIB (JH25073) (for exact genotypes and source of strains see table I). The strains had been grown in sporulation medium under conditions that have beenMol Microbiol. Author manuscript; available in PMC 2013 January 27.Fukushima et al.Pagereported to induce filamentation and development cessation. Especially, filamentation and growth cessation was observed for the divIC PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/23907221 and divIB strains when shifting the development temperature from 30 to 37 for 180 min incubation or from 37 to 48 for 90 min, respectively.F any observed interactions, a different B. subtilis kinase, ResE was also
F any observed interactions, yet another B. subtilis kinase, ResE was also probed for interaction together with the cell division proteins. The ResE kinase could be the closest B. subtilis homolog to YycG with 30 (51) identical (equivalent) residues more than the complete length on the protein and characteristics an identical domain architecture. When co-expressing all achievable combinations of the above pointed out fusion proteins (Fig. 5) six observations may be produced. (1) YycG, YycG211-611 and ResE homodimerized as expected for sensor kinases. (two) Surprisingly ResE and YycG heterodimerized in 1 but not the other vector combination. We assume this to become an artifact. (three) YycG and YycG211-611 but not ResE interact strongly with FtsL, DivIB and Pbp2B in each vector combinations.
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